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3D Diffraction Tomography – MicroED

November 24, 2025 by

3D diffraction tomography uses a Precession Electron Diffraction (PED) acquired tilt series of 2D off-zone patterns from a nanocrystal and transforms them into a 3D diffraction volume. Automatic clustering and manual routines are then used to determine the unit cell parameters. The extracted intensities can then be used find a structural solution, using direct methods, producing a three-dimensional atomic structure of the crystal.

DigiSTAR–precession electron diffraction (PED) device enables the collection of quasi-kinematical intensities (X-Ray like) in any TEM. PED in combination with powerful software (3D difffraction tomography) enables the reconstruction of the reciprocal cell of any nanomaterial and the automatic measurement of the reflection intensities.

In this way you can use your TEM as a powerful tool to solve any nanocrystal structure with performance comparable to the most advanced Synchrotron beamlines.

For more information …

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